",$n;
 
Home
Sobre o LGE
Projetos
Ferramentas
Time do LGE
Imprensa
Publicações
Links
Como chegar
Contato
Intranet
Webmail
Bioinformática
 





 
Genome structure of a Saccharomyces cerevisiae strain widely used in bioethanol production.   Argueso, J. L.; carazzolle, M. F.; Mieczkowski, P. A.; Duarte, F. M.; Netto, O. V. C.; Missawa, S. K.; Galzerani, F.; Costa, G. G. l.; Vidal, R. O.; Noraonha, M. F.; Dominska, M.; Andrieta, M. G. S.; Andrietta, S. R.; Cunha, A. F.; Gomes, L. H.; Tavares, F. C. A.; Alcarde, A. R.; Dietrich, F. S.; Petes, T. D.; Pereira, G. A. G.  Genome Research, dezembro de 2009. 19:2258-2270. doi:10.1101/gr.091777.109  2009


Bioethanol is a biofuel produced mainly from the fermentation of carbohydrates derived from agricultural feedstocks by the yeast Saccharomyces cerevisiae. One of the most widely adopted strains is PE-2, a heterothallic diploid naturally adapted to the sugar cane fermentation process used in Brazil. Here we report the molecular genetic analysis of a PE-2 derived diploid (JAY270), and the complete genome sequence of a haploid derivative (JAY291). The JAY270 genome is highly heterozygous (approximately 2 SNPs/kb) and has several structural polymorphisms between homologous chromosomes. These chromosomal rearrangements are confined to the peripheral regions of the chromosomes, with breakpoints within repetitive DNA sequences. Despite its complex karyotype, this diploid, when sporulated, had a high frequency of viable spores. Hybrid diploids formed by outcrossing with the laboratory strain S288c also displayed good spore viability. Thus, the rearrangements that exist near the ends of chromosomes do not impair meiosis, as they do not span regions that contain essential genes. This observation is consistent with a model in which the peripheral regions of chromosomes represent plastic domains of the genome that are free to recombine ectopically and experiment with alternative structures. We also explored features of the JAY270 and JAY291 genomes that help explain their high adaptation to industrial environments, exhibiting desirable phenotypes such as high ethanol and cell mass production and high temperature and oxidative stress tolerance. The genomic manipulation of such strains could enable the creation of a new generation of industrial organisms, ideally suited for use as delivery vehicles for future bioenergy technologies.
 
<< Voltar