Comparative analyses of the complete genome sequences of Pierce's disease and citrus variegated chlorosis strains of Xylella fastidiosa.
M.A. Van Sluys, M.C. de Oliveira, C.B. Monteiro-Vitorello, C.Y.
Miyaki, L.R. Furlan, L.E.A. Camargo, A.C.R. da Silva, D.H. Moon, M.A.
Takita, E.G.M. Lemos, M.A. Machado, M.I. . Ferro, F.R. da Silva,
M.H.S. Goldman, G.H. Goldman, M.V.F. Lemos, H. El-Dorry, S.M. Tsai, H.
Carrer, D.M. Carraro, R.C.de Oliveira, L.R. Nunes, W.J. Siqueira, L.L.
Coutinho, E.T. Kimura, E.S. Ferro,14 R. Harakava, E.E. Kuramae, C.L.
Marino, E. Giglioti, I.L. Abreu, L.M.C. Alves, A.M. do Amaral, G.S.
Baia, S.R. Blanco, M.S. Brito, F.S. Cannavan, A.V. Celestino, A.F. da
Cunha, R.C. Fenille, J.A. Ferro, E.F. Formighieri, L.T. Kishi, S.G.
Leoni, A.R. Oliveira, V.E. Rosa Jr., F.T. Sassaki, J.A.D. Sena, A.A.
de Souza, D. Truffi, F. Tsukumo, G.M. Yanai, L.G. Zaros, E.L.
Civerolo, A.J.G. Simpson, N.F. Almeida Jr., J.C. Setubal, and J. P.
Kitajima. Journal of Bacteriology, v. 185, n. 3, p. 1018-1026. 2003
Xylella fastidiosa is a xylem-dwelling, insect-transmitted,
gamma-proteobacterium that causes diseases in many plants, including
grapevine, citrus, periwinkle, almond, oleander, and coffee. X.
fastidiosa has an unusually broad host range, has an extensive
geographical distribution throughout the American continent, and
induces diverse disease phenotypes. Previous molecular analyses
indicated three distinct groups of X. fastidiosa isolates that were
expected to be genetically divergent. Here we report the genome
sequence of X. fastidiosa (Temecula strain), isolated from a naturally
infected grapevine with Pierce's disease (PD) in a wine-grape-growing
region of California. Comparative analyses with a previously sequenced
X. fastidiosa strain responsible for citrus variegated chlorosis (CVC)
revealed that 98% of the PD X. fastidiosa Temecula genes are shared
with the CVC X. fastidiosa strain 9a5c genes. Furthermore, the average
amino acid identity of the open reading frames in the strains is
95.7%. Genomic differences are limited to phage-associated chromosomal
rearrangements and deletions that also account for the strain-specific
genes present in each genome. Genomic islands, one in each genome,
were identified, and their presence in other X. fastidiosa strains was
analyzed. We conclude that these two organisms have identical
metabolic functions and are likely to use a common set of genes in
plant colonization and pathogenesis, permitting convergence of
functional genomic strategies.
Apoio financeiro: FAPESP, CNPq e USDA-ARS