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Multiple overlapping positions of nucleosomes with single rotational setting in the Hansenula polymorpha MOX promoter   Constanzo, G., Di Mauro, E., Negri, R., Pereira, G.A.G. and Hollenberg, C.P.  J. Biol. Chem. 270: 11091-11097  1995


In vivo nucleotide-level mapping of nucleosomes in the promoter of the methanol oxidase (MOX) gene in the yeast Hansenula polymorpha is reported. The 4 nucleosomes analyzed are organized in families; they localize in alternative positions along a unique rotational phase, and the linker regions can be occupied by alternative nucleosomes. This organization underscores a substantial freedom of choice by histone octamers when nucleating on a promoter region.


Apoio financeiro: Fundação Konrad-Adenauer, CNPq
 
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